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  Singlet oxygen, molecular oxygen in the lowest excited state, has a critical role in the cell-killing mechanism of photohynamic therapy (PDT). Although singlet oxygen phosphorescence measurement has been mainly used to monitor singlet oxygen formation during PDT, its intensity is far insufficient to obtain two-dimensional images of intracellular singlet oxygen with the subcellular spatial resolution using the currently available near-IR detector. Here, we propose a new far-red fluorescence probe of singlet oxygen, namely, Si-DMA, composed of silicon-containing rhodamine and anthracene moieties as a chromophore and a singlet oxygen reactive site, respectvily. For the first time, we successfully visualized singlet oxygen generated during PDT with a spatial resolution of a single mitochondrial tubule.